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Lonneke (L) Wigman

Department: Experimental Immunohematology

E-mail: l.wigman@sanquin.nl

Training: Medical Biology, Radboud Universiteit Nijmegen.

Research interests: Genetic basis of red cell high frequency antigens.
HFA are expressed by >99% of the donors. Currently, if HFA-negative red cells are needed, transfusion will be delayed for several hours, because it takes time to select, thaw and ship a unit. The availability of HFA-negative red cells is, also within the inventory of frozen units, limited, therefore only in case of transfusion urgency the minimal requested number of frozen red cells are provided. A completely typed HFA-negative donor pool would not only improve the availability of frozen units, but even also of fresh units in cases of scheduled surgery or expected delivery. Serological typing for HFA is hampered by lack of robust typing reagents and genotyping is not in all cases possible because the molecular background is not known for all clinically relevant HFA. Our project aims to elucidate the molecular background of a set of HFA and thus to improve transfusion medicine by facilitating availability of genotype-identified HFA-negative red cells.

Technology: Flowcytometric analysis, Next generation sequencing, MLPA, Immunoprecipitation, Two-dimensional gel electrophoresis and Mass spectrometric analysis of protein

Resume
2009-presentPhD student, Department of Experimental Immunohematology, Sanquin Research, Amsterdam.
Research on the genetic basis of high frequency antigens
2008Traineeship, Department of Pathology, UMC St. Radboud, Nijmegen.
Research on the difference of specific markers between primary diffuse infiltrating gliomas and their recurrence(s).
2006-2007Traineeship, Department of Biomolecular Chemistry, Nijmegen Centre for Molecular Life Sciences, Nijmegen.
Research on the effect of known Cartilage Hair-Hypoplasia mutations on the formation of the RNase MRP complex.
2006Traineeship, Department of Farmacology and Toxicology and department of Human Genetics, Nijmegen Centre for Molecular Life Sciences and UMC St. Radboud, Nijmegen.
Research on the effect of a polymorphism in the promoter region of Heme oxygenase-1 in rheumatoid arthritis.

Publications

Other publications
Wagener FA, Toonen EJ, Wigman L, Fransen J, Creemers MC, Radstake TR, Coenen MJ, Barrera P, Van Riel PL and Russel FG. HMOX1 promoter polymorphism modulates the relationship between disease activity and joint damage in rheumatoid arthritis. Arthritis Rheum 2008; 58(11):3388-93.



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